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To investigate whether PLSCR1 plays an important role in TLR9 and CpGDNA trafficking in pDCs, We analyzed the localization of TLR9 and CpGDNA in the EE in pDCs from wildtype mice and PLSCR1deficient mice following arousal with CpGA. We found that at 5 h following CpGA fun, CpGA was colocalized with PLSCR1 and TLR9 around the EEs in pDCs from wildtype mice (person 5B). however,within the other hand, In pDCs from PLSCR1deficient killing of mice, Only CpGADNA but not TLR9 was throughout the EEs.
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