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The ELISA guides (Rapidbio, american) Were solid phase enzyme immunoassays based on a twostep sandwich format and measured both the latent and active forms of the enzymes. A test run was performed for each assay to determine the appropriate dilution factor of 100 of each collected sample culture medium to ensure that the readings correlated with the exponential part of the respective standard curves. To eliminate the potential for interassay variation for each ELISA, The biological samples were assayed in one run.
Three harvests on sequential days are thus obtained, followed by,christian louboutin pigalle, End of manufacturing (EOP) VPCs are saved. Each harvest is processed by stepfiltration on the day of collection and transmitted into a second sterile 5 l bioprocessing bag upon filtration as previously described8 using a peristaltic pump (250 ml/min). The two first bounty are stored at 4 until pooling.
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