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TRFLP and clone librariesFor TRFLP,mcm taschen, PCR amplification of bacterial DNA extracted from all 36 of the sampled cryoconites was conducted with bacterial primers 27F (5 on fluorophore Cy5) And 1389R and looked at by capillary electrophoresis, After reduction digestion with HaeIII. Primers exact for archaea (Arch021F and consequently methanogenic archaea (McrAF were also screened. Detailed methodology for TRFLP and PCR with archaeaspecific primers is given in extra Data S1,
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One proposed mechanism for focal adhesion induction by Shigella is association of the IpaB complex with the 51 integrin (Watarai et ing, 1996). This alliance, to the, Does not appear to mediate appreciable binding of the bacterium to the cell surface (Watarai et ing, 1996) And is thus unlikely to allow bacterial internalization mediated by 1 integrins based on high affinity receptor relationships. will be possible, on the flip side,bridestowe lavender bear, That a combination of several signal transduction pathways is used by these pathogens during internalization by host cells.
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