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16.03.2014, 10:36

bridestowe bear nz 640

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Plasmid pGEM9Zf containing the cDNA region coding for FGF8 was kindly offered by Dr C Dickson (Viral Carcinogenesis clinical, ICRF, birmingham, uk, leeds). Plasmid DNA was linearized with endonuclease digestion of food using HindIII and BamHI. In vitro transcribing was carried out using SP6 RNA polymerase to generate the sense probe and T7 RNA polymerase to generate the antisense probe; Digoxigenin112' deoxyuridine 5'triphosphate was built into the riboprobes (Boehringer, britain).

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