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In each biotinylation reaction, 10 l of PCR product at 5 ng l1 was created in 20 l initial reaction volume (all the rest was 5 l TE buffer and 5 l Positive Control Mix, Which housed PCR product targets for human gene oligonucleotides printed on the microarray, As talked about in a previous study (Moisander et al, 2006)). previously hybridization, The biotinylated cures (hole) came denatured at 96 for 5 min, also 13.2 l target was added to the hybridization buffer employs 2 SSPE, 1% sodium dodecyl sulfate and 30% formamide (Final concentrations of mit) With 25 l final degree. The target was added on microarray slides that were engrossed in microarray cover slips and then hybridized at 50 for 18 h.